Abingdon, UK — A recently published article** from researchers at the Karolinska Institute details an improved protocol for cryopreservation of primary human hepatocyte using STEM-CELLBANKER?? cryopreservation solution from AMSBIO.
In the article the authors compare cryopreservation of liver hepatocytes using STEM-CELLBANKER??with the frequently used DMSO-University of Wisconsin (DMSO-UW) medium protocol. Both protocols yielded hepatocytes with good P450 function, however, the STEM-CELLBANKER??protocol gave a higher viability than the DMSO-UW cryoprotectant.
The researchers concluded that??STEM-CELLBANKER?? beneficially is also xeno-free and will be applicable to cryopreservation of clinical-grade primary human hepatocytes.
STEM-CELLBANKER????from AMSBIO is a chemically defined freezing medium with optimized formulation for stem cells and iPS cells storage as well as other valuable cells. Supplied ready-to-use with a simple usage protocol, STEM-CELLBANKER??is completely free of serum and animal derived components, and contains only European or US Pharmacopoeia graded ingredients.
Part of the CELLBANKER?? series of cell freezing media, STEM-CELLBANKER?? significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage.
Every batch of STEM-CELLBANKER?? cryopreservation medium is performance tested on Jurkat and SK-007 cells.
Additional standard evaluations for endotoxins, pH, osmolarity and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.
For further information on STEM-CELLBANKER?? please visit www.amsbio.com/STEM-CELLBANKER-Stem-Cell-Cryopreservation-Solution.aspx or contact AMSBIO on +44-1235-828200 / +1-949-768-8365 or email firstname.lastname@example.org.
** Saliem M et al. (2012) Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution. World J Hepatol. May 27;4(5):176-83.
AMS Biotechnology (AMSBIO), founded in 1987, is recognised as a leading international provider of unique, innovative products & custom services for life sciences research. The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, 3D culture, electrophoresis, glycobiology, post-translational modification & stem cell biology.
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